U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX19740773: Whole genome sequencing of Haloarcula hispanica DF60 (pyrF)
1 ILLUMINA (Illumina MiSeq) run: 471,271 spots, 142.3M bases, 69.5Mb downloads

Design: Culture was innoculated from a single colony into rich media (23% salt YPC with 50ug/mL uracil) and grown to mid-log phase at 37C. Genomic DNA was extracted using phenol-chloroform-isoamyl alcohol (25:24:1; Fisher Scientific) and ethanol precipitated. Purified DNA was quantified using a NanoDrop system (Thermo Scientific) and sonicated in a Diagenode sonicating water bath for 20 cycles on high. DNA quality was assessed by using a bioanalyzer using a high-sensitivity DNA chip (Agilent). Samples were submitted to Duke Center for Genomic and Computational Biology core sequencing facility for adapter ligation with TruSeq (Illumina) adapters and library amplification.
Submitted by: Duke University
Study: Haloarcula hispanica strain sequencing
PRJNA947196 • SRP428442 • All experiments • All runs
show Abstracthide Abstract
Whole genome sequencing of five Haloarcula hispanica strains to confirm expected genotypes and identify variants from RefSeq reference genome.
Sample: pyrF strain (Schmid lab derivative of Liu et. al. 2011a) whole genome sequencing
SAMN33844742 • SRS17106784 • All experiments • All runs
Organism: Haloarcula hispanica ATCC 33960
Library:
Name: Hh_DF60
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 471,271 spots, 142.3M bases, 69.5Mb
Run# of Spots# of BasesSizePublished
SRR23930647471,271142.3M69.5Mb2023-05-30

ID:
27057115

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...